human TNF-RII

SYNONYMS:Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor, , Etanercept, TBPII, TNFBR, TNFR80, TNF-R75, p75TNFR, TNF-R-II.

SOURCE:Chinese Hamster Ovarian Cells (CHO)

PURITY:>98% by SDS-PAGE and HPLC analysesEndotoxin level is <0.1 ng per ug (1EU/ug).

MOL. WEIGHT:15 kDa

FORM:Lyophilized from a sterile solution containing 1.6 mg mannitol, 0.4 mg sucrose and 48 ?g tromethamine per 1 mg protein.

RECONSTITUTION:Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers or store d at 4oC for 1 week or ?20oC for future use.

STORAGE CONDITIONS:The lyophilized protein is best-stored desiccated below 0oC. Reconstituted sTNF Receptor II should be stored at working aliquots at -20oC. BACKGROUND

DESCRIPTION:Tumor Necrosis Factor Receptor Type II is an inhibitor of TNF and can block TNF bioactivity. Recombinant human TNF-RII produced in CHO is a dimeric, glycosylated, polypeptide chain consisting of the extracellular ligand-binding portion of the human 75 kilodalton (p75) tumor necrosis factor receptor (TNFR) linked to the Fc portion of human IgG1. The Fc component of TNFRII contains the CH2 domain, the CH3 domain and hinge region, but not the CH1 domain of IgG1. It consists of 934 amino acids and has an apparent molecular weight of approximately 150 kilodaltons.

BIOLOGICAL ACTIVITY:Potency is determined by its ability to neutralize TNF-alpha mediated growth inhibition of A375 cells, corresponding to a Specific Activity of 1.7 x 107 IU/mg.

USAGE:

FOR RESEARCH USE ONLY! Not to be used in humans.