Human TNF-alpha ELISA Kit

分子 : TNF

 

抗原貨號 : 10602-HNAE

 

產品介绍 : For the quantitative determination of Human TNF-alpha concentration in cell culture supernates.

 

線性範圍: 31.25-2000

 

包被抗體類型 : mouse mAb

 

檢測抗體類型 : mouse mAb

 

靈敏度: 13.06

 

天然樣本測定含量: Human peripheral blood mononuclear cells (2 x 10E6 cells/mL) were cultured in DMEM supplemented with 5% bovine calf serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate, stimulated with 1 μg/mL PHA for 5 days. Aliquots of the cell culture supernates were removed and assayedfor levels of natural TNFa, and measured 1.14 ng/mL.

 

特異性 : This assay recognizes both recombinant and natural Human TNFa. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

 

干擾 : Preparations of the following factors at 50 ng/mL in a mid-range recombinant Human TNFa control were assayed for interference. No significant interference was observed.

 

檢測原理: Solid Phase Sandwich ELISA

 

試劑盒组分 :

1. 96 well microplate coated with Capture Antibody

2. Detection Antibody conjugated to HRP

3. Standards

4. Wash Buffer Concentrate

5. Dilution Buffer Concentrate

6. Color Reagent A

7. Color Reagent B

8. Stop Solution