Human TIMP-2 Protein, His Tag

Source : 

Human TIMP-2, His Tag (TI2-H5223) is expressed from human 293 cells (HEK293). It contains AA Cys 27 - Pro 220 (Accession # NP_003246.1).

 

Molecule : TIMP-2

 

Synonyms : CSC-21K,TIMP2

 

Format : Powder

 

Category : Other Recombinant Proteins

 

Accession : NP_003246.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 22.6 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 22.6 kDa. The protein migrates as 23-26 kDa under reducing (R) condition (SDS-PAGE).

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 50 mM Tris, 150 mM NaCl, pH7.5

 

Description :

TIMP metallopeptidase inhibitor 2 is also known as TIMP2, which belongs to the protease inhibitor I35 (TIMP) family. This family protein are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The TIMP family encompasses four members ( TIMP1, TIMP2, TIMP3, TIMP4 ), and they inhibit most MMPs by forming non-covalent binary complex. In addition to an inhibitory role against metalloproteinases, TIMP2 has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, TIMP-2 may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix.

 

References :

(1) Stetler-Stevenson W.G., et al., 1989, J. Biol. Chem. 264:17374-17378.
(2) Goldberg G.I., et al., 1989, Proc. Natl. Acad. Sci. U.S.A. 86:8207-8211.
(3) Chattopadhyay N., et al., 2001, J. Cancer Res. Clin. Oncol. 127:653-658.