Human TIMP-1 / TIMP1 ELISA Kit

分子 : TIMP1

 

抗原貨號 : 10934-H08H

 

產品介绍 : For the quantitative determination of TIMP-1 / TIMP1 concentration in serum and saliva.

 

線性範圍: 7.8-500

 

包被抗體類型 : mouse mAb

 

檢測抗體類型 : rabbit pAb

 

靈敏度: 3.13

 

天然樣本測定含量: The average concentration of Human TIMP1 in 10 normal human serum is 131.79 +/- 28.86 ng/mL ranging from 87.24 to 172.97 ng/mL and in 5 normal salvia is 363.10 +/- 131.44 ng/mL ranging from 239.42 to 550.67 ng/mL.

 

特異性 : This assay recognizes both recombinant and natural Human TIMP1. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed. Cross-reactivity was observed to be 0.086% with 50 ng/mL of recombinant human MMP9.

 

干擾 : Preparations of the following factors at 50 ng/mL in a mid-range recombinant
human TIMP1 control were assayed for interference. No significant interference was observed.

 

檢測原理: Solid Phase Sandwich ELISA

 

試劑盒组分 :

1. 96 well microplate coated with Capture Antibody

2. Detection Antibody conjugated to HRP

3. Standards

4. Wash Buffer Concentrate

5. Dilution Buffer Concentrate

6. Color Reagent A

7. Color Reagent B

8. Stop Solution