Human TIGIT Protein, Mouse IgG2a Fc Tag, low endotoxin

Source : 

Human TIGIT, Mouse IgG2a Fc Tag (TIT-H5253) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Pro 141 (Accession # Q495A1-1).

 

Molecule : TIGIT

 

Synonyms : TIGIT,VSIG9,VSTM3

 

Format : Powder

 

Category : Immune Checkpoint Proteins

 

Accession : AAI01290.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 40.1 kDa

 

Characteristics :

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 40.1 kDa. The protein migrates as 42-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 0.1 EU per μg by the LAL method.

 

Buffer : Tris with Glycine, Arginine and NaCl, pH7.5

 

Description :

TIGIT (also called T cell immunoreceptor with Ig and ITIM domains) is one newly discovered immune receptor on some percentage of T cells and Natural Killer Cells(NK). It is also identified as WUCAM and Vstm3. TIGIT could bind to CD155(PVR) on dendritic cells(DCs), macrophages, etc. with high affinity, and also to CD112(PVRL2) with lower affinity. TIGIT expressing CD8+ T cells has been shown to be expanded and associated with clinical markers of HIV disease progression in a diverse group of HIV infected individuals. TIGIT and PD-1 has been shown to be over expressed on tumor antigen-sepcific (TA-specific) CD8+ T cells and CD8+ tumor infiltrating lymphocytes (TILs) from individuals with melanoma. Blockade of TIGIT and PD-1 led to increased cell proliferation, cytokine production, and degranulation of TA-specific CD8+ T cells and TIL CD8+ T cells.

 

References :

(1)Chew GM., et al., 2016, PLoS Pathogens., 12: e1005349.
(2)Joe-Marc Chauvin, et al., 2015, J Clin Invest., 125 (5): 2046-2058.
(3)Steven G. Deeks, 2012, Nature, 487 (1): 439-440.