Human TIGIT Protein, His Tag (MALS verified)

Source : 

Human TIGIT, His Tag (MALS verified) (TIT-H52H3) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Pro 141 (Accession # Q495A1-1).

 

Molecule : TIGIT

 

Synonyms : TIGIT,VSIG9,VSTM3

 

Format : Powder

 

Category : Immune Checkpoint Proteins

 

Accession : AAI01290

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 14.9 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 14.9 kDa. The protein migrates as 17-21 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

 

References :

(1) Yu X., et al., 2009, Nat. Immunol. 10:48-57.
(2) Stengel K.F., et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109:5399-5404.
(3) Lozano E., et al., 2012, J. Immunol. 188:3869-3875.