Human Thioredoxin 1 (Trx1) Inhibitor Screening Kit (Fluorometric)

Alternate Name :

Tag Line :

Simplest High-Throughput Assay To Screen Thioredoxin 1 Inhibitors

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method-Fluorescence (Ex/Em: 490/525 nm)
• Applications- Screening/studying/characterizing Thioredoxin 1 inhibitors

Detection Method: Fluorescence (Ex/Em: 490/525 nm)

Features & Benefits:

• Rapid, simple & sensitive
• Convenient & HTS
• Includes inhibitor control

Description:

The human Thioredoxin 1 (Trx1) is a key enzyme that protects cells from oxidative stress by reducing disulfides and methionine sulfoxide groups in oxidized proteins. It also regulates DNA repair and apoptosis. In mammals, Trx1 is necessary for growth and development. However, an aberrant elevated expression of Trx1 is known to promote lung, cervical, pancreatic, colorectal, and breast cancers. In BioVision’s Trx1 Inhibitor Screening Kit, Trx1 reduces quenched di-FITC-labeled oxidized glutathione (Trx1 substrate), to mono-FITC-labeled unquenched reduced glutathione. In the presence of a Trx1 inhibitor (control inhibitor included), the reaction is impeded resulting in lower or no signal. The assay is high-throughput adaptable and can be completed in less than 1 hr