Human Stathmin 1 / STMN1 Protein, His Tag

Source : 

Human Stathmin 1, His Tag (ST1-H5149) is expressed from E.coli cells. It contains AA Ala 2 - Asp 149 (Accession # NP_981946).

 

Molecule : Stathmin 1

 

Synonyms : STMN1,Stathmin,C1orf215,LAP18,OP18

 

Format : Powder

 

Category : Other Recombinant Proteins

 

Accession : NP_981946

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 18.1 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 18.1 kDa. The protein migrates as 18 kDa under reducing (R) condition (SDS-PAGE).

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Stathmin (STMN1), a member of the stathmin family, is also known as Leukemia-associated phosphoprotein p18, Oncoprotein 18 (Op18), Phosphoprotein p19 (pp19), Protein Pr22 and pp17, which contains one SLD (stathmin-like) domain. The function of STMN1 as an important regulatory protein of microtubule dynamics has been well-characterized. Stathmin (STMN1) interacts with two molecules of dimeric α,β-tubulin to form a tight ternary complex called the T2S complex.One mole of STMN1 binds to two moles of tubulin dimers through the stathmin-like domain (SLD).When STMN1 sequesters tubulin into the T2S complex, tubulin becomes non-polymerizable. Without tubulin polymerization, there is no microtubule assembly.STMN1 also promotes microtubule disassembly by acting directly on the microtubule ends.

 

References :

(1) Jourdain L., et al., 1997, Biochemistry. 36(36):10817-21.
(2) Rubin CI., et al., 2004, J Cell Biochem. 93(2):242-50.
(3) Redeker V., et al., 2000, J. Biol. Chem. 275:6841-6849.