Human Sonic Hedgehog / Shh Protein, His Tag

Source : 

Human Sonic Hedgehog, His Tag (SH7-H5229) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Gly 197 (Accession # NP_000184).

 

Molecule : Sonic Hedgehog

 

Synonyms : SHH,HHG-1,Sonic hedgehog,HLP3,HPE3,SMMCI,TPT,TPTPS

 

Format : Powder

 

Category : Cytokines&Growth Factors

 

Accession : NP_000184

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 20.4 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.4 kDa. The protein migrates as 23-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Sonic hedgehog protein (SHH) is also known as HHG-1, which belongs to the hedgehog family. SHH is expressed in fetal intestine, liver, lung, and kidney and not expressed in adult tissues. Sonic hedgehog protein can be cleavage into sonic hedgehog protein N-product and sonic hedgehog protein C-product by autolysis. SHH binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development. The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.

 

References :

(1) Pepinsky R.B., et al., 1998, J. Biol. Chem. 273:14037-14045.
(2) Marigo V., et al., 1995, Genomics 28 (1): 44–51.
(3) Echelard Y., et al., 1993, Cell 75 (7): 1417–30.