Human Soluble Vascular Endothelial Growth Factor receptor 1 / sVEGFR1 ELISA Kit (1 plate)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

vascular permeability factor receptor; Tyrosine-protein kinase receptor FLT; Tyrosine-protein kinase FRT; Fms-like tyrosine kinase 1; VEGFR-1; FLT1;

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1454

 

Description (Long Description) : 

The human Vascular Endothelial Growth Factor receptor 1 (VEGFR1) Kit is a sandwich ELISA. The capture antibody is a monoclonal human VEGFR1 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known VEGFR1 concentration are added to these wells and allowed to complex with the bound VEGFR1 antibody. A biotinylated anti-human VEGFR1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of VEGFR1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human VEGFR1 in samples such as sera, plasma, and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

 

Expiry_Date : 

This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :Recombinant sVEGFR1 amino acids 27-687 (located in the ECD domain region) produced in and purified from a sf21 insect culture system.

 

Kit_Components : The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, detection reagents, substrate buffer and detailed protocols.

 

MSDS (File name) :