Human Soluble ICAM-1 (sICAM-1) ELISA Kit (1 plate)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

ICAM1; ICAM-1; Intercellular adhesion molecule 1; CD54;

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info : Intercellular adhesion molecule 1 (ICAM1) is a single-pass type 1 membrane glycoprotein that belongs to the ICAM family. ICAM1 is typically expressed on endothelial cells and cells of the immune system.

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1334

 

Description (Long Description) : 

The human ICAM-1 Kit is a sandwich ELISA. The capture antibody is a monoclonal human ICAM-1 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known ICAM-1 concentration are added to these wells and allowed to complex with the bound ICAM-1 antibody. A biotinylated human ICAM-1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of ICAM-1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human ICAM-1 in samples such as sera, plasma, and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

 

Expiry_Date : 

This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids Q28-E480

 

Kit_Components : The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.

 

MSDS (File name) :