Human SMAC / Diablo Protein, His Tag

Source : 

Human SMAC, His Tag (DIO-H5129) is expressed from E.coli cells. It contains AA Ala 56 - Asp 239 (Accession # NP_063940).

 

Molecule : SMAC

 

Synonyms : DIABLO,SMAC

 

Format : Powder

 

Category : Other Recombinant Proteins

 

Accession : NP_063940

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 21.7 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21.7 kDa. The protein migrates as 22 kDa under reducing (R) condition (SDS-PAGE).

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 20 mM HEPES, 150 mM NaCl, pH7.5

 

Description :

Diablo homolog, mitochondrial (DIABLO) is also known as direct IAP-binding protein with low pI and second mitochondria-derived activator of caspase (SMAC), which can interact with BIRC2/c-IAP1, BIRC3/c-IAP2, XIAP/BIRC4, BIRC6/bruce and BIRC7/livin. Acting by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP),DIABLO can also promote apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Also, DIABLO inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Furthermore, DIABLO is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.

 

References :

(1) Du C., et al., 2000, Cell 102:33-42.
(2) Fu J., et al., 2003, J. Biol. Chem. 278:52660-52672
(3) Ma L., et al., 2006, Cell Death Differ. 13:2079-2088.