Human Siglec-8 Protein, Fc Tag

Source : 

Human Siglec-8, Fc Tag (SI8-H5254) is expressed from human 293 cells (HEK293). It contains AA Met 17 - Ala 363 (Accession # Q9NYZ4-1).

 

Molecule : Siglec-8

 

Synonyms : Siglec-8,SAF-2,SIGLEC8,SAF2

 

Format : Powder

 

Category : Other Recombinant Proteins

 

Accession : NP_055257.2

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 64.3 kDa

 

Characteristics :

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 64.3 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Siglec-8 is also known as SIGLEC8, SAF2, SIGLEC-8, SIGLEC8L and sialic acid binding Ig like lectin 8. Siglec-8 was first identified by CD33 homology screening of ESTs from a cDNA library generated from a patient diagnosed with idiopathic hypereosinophilic syndrome and was originally termed SAF-2 (sialoadhesin family 2). At the tissue level, Siglec-8 mRNA was found to be most highly expressed in lung, PBMCs, spleen, and kidney. Two splice variants of Siglec-8 exist. The initially characterized form contains 431 amino acid residues in total, subsequently, a longer form of Siglec-8, initially termed Siglec-8L. Both forms of Siglec-8 are found in eosinophils and contain a V-set domain with lectin activity and two C2-type Ig repeat domains in the extracellular region.

 

References :

(1) Floyd H, et al. 2000. J Biol Chem. 275(2):861-6.
(2) Foussias G, et al. 2000. Biochem Biophys Res Commun. 278(3):775-81.
(3) Kikly KK, et al. 2000. J Allergy Clin Immunol. 105(6 Pt 1):1093-100.