Human Siglec-10 Protein, His Tag

Source : 

Human Siglec-10, His Tag (SI0-H52H9) is expressed from human 293 cells (HEK293). It contains AA Met 17 - Thr 546 (Accession # Q96LC7-1).

 

Molecule : Siglec-10

 

Synonyms : SIGLEC10,MGC126774,PRO940,Siglec10,SLG2

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : NP_149121.2

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 60.1 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 60.1 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 25 mM MES, 150 mM NaCl, Arginine, pH5.5

 

Description :

The siglecs (sialic acid-binding Ig-like lectins) are a distinct subset of the Ig superfamily with adhesion-molecule-like structure. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.

 

References :

(1) Whitney G., et al. 2001, Eur J Biochem., 268(23), 6083-96.
(2) Kivi E., et al. 2009, Blood., 114(26), 5385-92.
(3) Munday J., et al. 2001, Biochem J., 355(Pt 2), 489-97.