Human Sex Hormone Binding Glob (SHBG) ELISA Kit

SpeciesReactivity

Human

Samples

Serum. SHBG in culture medium (LNCaP, DU 145, and PC 3 cells) has also been used (see published references). Plasma, Culture medium and other fluids may be adapted

KitContents

1. Mouse Anti-h SHBG coated microwell strip plate (96 wells). Ready-to-use 701 2. SHBG Standard A, 0 nmol/L, 16 ml 702 3. SHBG Standard B, 3.3 nmol/L, 0.4 ml 703 4. SHBG Standard C, 12.5 nmol/L, 0.4 ml 704 5. SHBG Standard D, 55 nmol/L, 0.4 ml 705 6. SHBG Standard E, 160 nmol/L, 0.4 ml 706 7. SHBG Standard F, 295 nmol/L, 0.4 ml 707 8. SHBG Control, 0.4 ml (value printed on the vial) 700C Lot Specific values of standards are printed on each vial. They should be used for plotting std. Curve. Calibrated against WHO 1st IS 95/560 9. Assay Buffer, 40 ml 708 10. Anti-hSHBG-HRP Conjugate (50 X soln), 0.4 ml 709 11. Wash buffer concentrate (10X solution) 12. 50 ml (dilute to 500 ml in distilled water) W-10 13. HRP substrate soln, 16 ml S-20 14. Stop solution, 6 ml T-30 15. Complete Instruction Manual M-700

Form

Stds=0-300 nmol/L; Sample=20 ul; Sensitivity= 0.2 nmol/L; 60 min assay; Quantitative, Sandwich, 96 tests

SpCrossreactivity

Human SHBG ELISA kit is specific for human SHBG with no significant reactivity TBG or other serum proteins. Human SHBG ELISA kit species crossreactivity with TSH from other species (mouse, rat, and monkey) has not been established.

MSDS

TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates