Human Serpin D1 / Heparin Cofactor II Protein, His Tag

Source : 

Human Serpin D1, His Tag (HC2-H5224) is expressed from human 293 cells (HEK293). It contains AA Gly 20 - Ser 499 (Accession # AAH35028).

 

Molecule : Serpin D1

 

Synonyms : SerpinD1,HCF2,HLS2,HC-II

 

Format : Powder

 

Category : Other Recombinant Proteins

 

Accession : AAH35028

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 55.7 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 55.7 kDa. The protein migrates as 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 50 mM Tris, 150 mM NaCl, pH 7.5

 

Description :

Serpin D1 is also known as Heparin cofactor 2 (HCF2), Protease inhibitor leuserpin-2 (HLS2), Heparin cofactor II (HC-II), which belongs to the serpin family. HCF2 is expressed predominantly in liver. Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, SerpinD1 / HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). In a glycosaminoglycan-independent manner, serpinD1 / HC-II also inhibits chymotrypsin. Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils.

 

References :

(1) van Deerlin V.M.D., et al., 1991, J. Biol. Chem. 266:20223-20231.