Human Rheumatoid Factors IgM (RF) ELISA Kit

SpeciesReactivity

Human

Samples

Serum. Other fluids not optimized.

KitContents

1. Purified gamma globulin coated microwell strips (96 wells), Ready-to-use P-3001 2. RF IgM Negative Control (100X), 0.3 ml 3002 3. RF IgM Positive Standard (100X), 0.3 ml 3003 4. RF IgM Calibrator (100X), 0.3 ml 3004 5. Sample Diluent, 50ml 3005 6. Anti-hIgM HRP Conjugate, 11 ml 3006 7. Wash Buffer Concn. (20X solution), 50 ml 3007 8. HRP Substrate Buffer, Solution A; 11 ml 3000SA 9. HRP Substrate, Solution B, 11 ml 3000SB 10. Stop solution, 10 ml T-10 11. Complete Instruction Manual M-3000

Form

Stds=0, 25, 75 IU/ml calibrators; Sample=100 ul; 70 min; Semi-Quant., Direct Antigen assay

SpCrossreactivity

Human Rheumatoid Factors IgM (RF) ELISA kit is specific for human. A similar kit for mouse is also available.

MSDS

TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates