Human Renin ELISA Kit (1 Plate)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Angiotensinogenase

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

ELISA. The purpose of this kit is the in-vitro quantitative determination of Human Renin protein in samples such as cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info : Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1694

 

Description (Long Description) : 

The human Renin kit is a sandwich ELISA. The capture antibody is a mouse monoclonal human Renin antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known human Renin concentration are added to these wells and allowed to complex with the bound human Renin antibody. A biotinylated human Renin goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Renin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Renin in samples such as cell culture supernatants, serum, plasma (heparin, EDTA) and urine. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

 

Expiry_Date : 

6 months after purchase if unopened

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :The standard and immunogen for this kit is recombinant Human Renin expressed in a NSO expression system; Immunogen sequence: L67-R406

 

Kit_Components : The ELISA kit box contains 1 x 96-well pre-coated strip plate, Human Renin protein standards, Human Renin biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.

 

MSDS (File name) :http://www.biosensis.com/pdf_MSDS.php?products_id=1694