Human RANK / TNFRSF11A Protein, Mouse IgG2a Fc Tag, low endotoxin

Source : 

Human RANK, Mouse IgG2a Fc Tag, low endotoxin (RAK-H5251) is expressed from human 293 cells (HEK293). It contains AA Ile 30 - Pro 212 (Accession # Q9Y6Q6-1).

 

Molecule : RANK

 

Synonyms : CD265,FEO,LOH18CR1,ODFR,OFE,OPTB7,OSTS,PDB2,RANK,TNFRSF11A,TRANCER,CD265 antigen,ODFROSTS,RANKLOH18CR1,NFKB activator

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : NP_003830.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 47.2 kDa

 

Characteristics :

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 47.2 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 0.1 EU per μg by the LAL method.

 

Buffer : Tris with Glycine, Arginine and NaCl, pH7.5

 

Description :

RANK / TNFRSF11A is a member of the tumor necrosis factor receptor family. RANK / TNFRSF11A shares significant amino acid homology with other members of the TNF R family in its extracellular four cysteine-rich repeats. Functional genomics in mice showed that RANKL (TNFSF11), a member of the TNF ligand superfamily, and RANK (TNFRSF11a), the cognate TNFR family receptor for RANKL, were essential for osteoclastogenesis in vivo. A functional interaction between RANKL, expressed by bone stromal cells of the osteoblast lineage, and RANK, expressed by osteoclast precursors of hematopoietic myeloid lineage, is necessary for osteoclast differentiation, survival, and activation.

 

References :

(1) Anderson DM., et al. 1997, Nature, ;390(6656), 175-9.
(2) Tomoyasu A., et al. 1998, Biochem Biophys Res Commun., 245(2),382-7.
(3) Dougall WC., et al. 2012, Clin Cancer Res.,18(2), 326-35.