Human RAGE / AGER Protein, Fc Tag

Source : 

Human RAGE, Fc Tag (AGR-H5253) is expressed from human 293 cells (HEK293). It contains AA Ala 23 - Ala 344 (Accession # NP_001127).

 

Molecule : RAGE

 

Synonyms : AGER,RAGE

 

Format : Powder

 

Category : Other Recombinant Proteins

 

Accession : NP_001127

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 60.8 kDa

 

Characteristics :

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 60.8 kDa. The protein migrates as 80 - 90 kDa and 35 kDa Fc fragment under reducing (R) condition (SDS-PAGE) due to glycosylation and auto-cleavage.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 50 mM Tris, 100 mM Glycine, pH7.5

 

Description :

Advanced glycosylation end product-specific receptor (AGER) is also known as RAGE, and is a single-pass type I membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. AGER is highly expressed in endothelial cells. AGER mediates interactions of advanced glycosylation end products (AGE), and also accumulates in vascular tissue in aging and at an accelerated rate in diabetes, as well as acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGER / RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes.

 

References :

(1) Fang F., et al., 2010, FASEB J. 24:1043-1055
(2) Jin Q., et al., 2011, PLoS ONE 6:E19375-E19375
(3) Xue J., et al., 2011, Structure 19:722-732