Human PSME3 / PA28g Protein, His Tag

Source : 

Human PSME3, His Tag (PS3-H5142) is expressed from E.coli cells. It contains AA Ala 2 - Tyr 254 (Accession # P61289-1).

 

Molecule : PSME3

 

Synonyms : PSME3,PA28gamma,REG-gamma

 

Format : Powder

 

Category : Other Recombinant Proteins

 

Accession : NP_005780.2

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 30.3 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 30.3 kDa. The protein migrates as 28-33 kDa under reducing (R) condition (SDS-PAGE).

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 50 mM Tris, 150 mM NaCl, pH7.5

 

Description :

Proteasome activator complex subunit 3 (PSME3),a member of the PA28 family, is also known as 11S regulator complex subunit gamma (REG-gamma), activator of multicatalytic protease subunit 3 and proteasome activator 28 subunit gamma (PA28gamma). PSME3 activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. PSME3 can also facilitate the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. Furthermore,PSME3 may also be involved in cell cycle regulation.

 

References :

(1) Miki Y., et al., 1994, Science 266:66-71.
(2) Realini C., et al., 1997,J. Biol. Chem. 272:25483-25492.
(3) Zhang Z., et al., 2008, EMBO J. 27:852-864.