Human PSGL-1 / CD162 Protein, His Tag

Source : 

Human PSGL-1, His Tag (PS1-H52H9) is expressed from human 293 cells (HEK293). It contains AA Gln 42 - Cys 320 (Accession # Q14242-1).

 

Molecule : PSGL-1

 

Synonyms : PSGL1,CD162,SELPLG,Selectin P ligand

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : NP_002997.2

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 30.7 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 30.7 kDa. The protein migrates as 55-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

P-selectin glycoprotein ligand 1 (PSGL-1) is also known as Selectin P ligand (SELPLG), CD antigen CD162. PSGL-1 is disulfide-linked homodimer which is the high affinitycounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 / SELPLG interacts with P-, E- and L-selectins, through their lectin/EGF domains, is required for promoting recruitment and rolling of leukocytes. These interactions require sialyl Lewis X glycan modification but there is a differing dependence for tyrosine sulfations. Dimerization appears not to be required for P-selectin/SELP binding. PSGL-1 can interact with SNX20, MSN and SYK. PSGL-1 / SELPLG mediate the activation of SYK by SELPLG.

 

References :

(1) Rodgers S.D., et al., 2001, Biophys. J. 81:2001-2009.
(2) Bernimoulin M.P., et al., 2003, J. Biol. Chem. 278:37-47.
(3) Somers W.S., et al., 2000, Cell 103:467-479.