Human Prothrombin (Factor II)

Prepared from fresh frozen human plasma. Human Prothrombin is a glycoprotein of molecular weight 72,000, and consists of a single polypeptide chain. Activation of Prothrombin by Factor Xa yields the serine protease Thrombin. Prothrombin is homogeneous as judged by 10% SDS-PAGE gels and shows no reduction upon incubation with 2-mercaptoethanol. Buffer composition = 20 mM Tris-HCl/0.1 M NaCl /1 mM Benzamidine/pH 7.4 *Extinction Coefficient (1%) = 13.6 One unit = 90 µg Molecular Weight =72,000 daltons.