Human Prolactin (PRL) ELISA Kit

SpeciesReactivity

Human

Samples

Serum. Culture medium has also been used (see published references). Plasma, Culture medium and other fluids may be adapted

KitContents

1. Anti-hProlactin coated strip plate (96 wells) , 1 plate 301 2. Prolactin Sample Diluent, or Std. A (0 ng/ml) 11ml SD-300A 3. Prolactin Std. B (5 ng/ml), 0.75 ml, 1 vial 303 4. Prolactin Std. C (10 ng/ml) 0. 75 ml, 1 vial 304 5. Prolactin Std. D (20 ng/ml) 0. 75 ml, 1 vial 305 6. Prolactin Std. E (50 ng/ml ) 0. 75 ml, 1 vial 306 7. Prolactin Std. F (100 ng/ml ) 0. 75 ml, 1 vial 307 Standards are calibrated to NIBSC 3rd prep. IS 84/500 8. Anti-hProlactin-HRP Conjugate 11 ml, 1 bottle 308 9. Wash buffer (20X), 50 ml (dilute 1:20 with water). Wb-20 10. Substrate Soln. A, 11 ml; 1 bottle 300SA 11. Substrate Soln. B, 11 ml; 1 bottle 300SB 12. Stop solution, 10 ml , 1 bottle T-10 13. Complete Instruction Manual M-300

Form

Stds=0-100 ng/ml ; Sample=25 ul; Sensitivity= 2 ng/ml; 45 min assay; Quantitative, Sandwich, 96 tests

SpCrossreactivity

Human prolactin ELISA kit is specific for human prolactin with no significant reactivity with LH, TSH, HCG or other peptide hormones. Human prolactin ELISA kit species crossreactivity with prolactin from other species (mouse, rat, and monkey) has not been established.

MSDS

TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates