Human Progesterone (saliva) ELISA Kit

SpeciesReactivity

Human

Samples

Serum. Other fluids not optimized.

KitContents

1. Anti-Progesterone Coated Strip plate, (96 wells) 1856 2. Progesterone Std. A, 10 ml; 0 pg/ml 1857A 3. Progesterone Std. B, 0.5; 20 pg/ml 1857B 4. Progesterone Std. C, 0.5 ml; 100 pg/ml 1857C 5. Progesterone Std. D, 0.5 ml; 500 pg/ml 1857D 6. Progesterone Std. E, 0.5 ml; 2000 pg/ml 1857E 7. Progesterone Std. F, 0.5 ml; 5000 pg/ml 1857F 8. Progesterone Control 0.5 ml 1867G Exact values of Stds and control (lot specific) are provided on the vials. Stability: 12 months in unopened vial or as indicated on label. Once opened, the control should be used within 14 days or aliquoted and stored frozen. Avoid multiple zing and thawing cycles. 9. Assay buffer, 15 ml 1858 10. Progesterone-HRP Conjugate, 300 ul (100X) 1859 11. Wash buffer 50 ml (10X) W-10 12. TMB Substrate Soln, 16 ml TMB-10 13. Stop Solution, 6 ml T-10 14. Complete Instruction Manual M-1855

Form

Stds=0-5000 pg/ml ; Sample=50 ul; Sensitivity= 20 pg/mlL; 75 min assay; Quantitative, Sandwich assay

SpCrossreactivity

The following compounds were tested for cross-reactivity with the Direct Progesterone ELISA kit with Progesterone cross-reacting at 100%. Progesterone (100%), 11-alpha-OH-Progesterone (100%), Deoxycorticosterone (1.7%), 17-OH-Progesterone, 5a-Androstan-3ß, 17ß-diol, Corticosterone, Pregnenolone, (<0.4%). The following steroids were tested but cross-reacted at less than 0.1%: Cortisol, Cortisone, Danazol, DHEAS, Estradiol, 5ß-Pregnan-3a, 17a, 21a-triol-20-one, 5ß-Pregnan-3a, 17-diol-20-one, Pregnan-3a, 20a-diol and Testosterone. Progesterone kit has been designed and tested for human serum samples. It may be optimized for other biological fluids. It has not been tested in animals (rat, mouse, etc). Since the steroid hormone is the same in all species, this kit should work in most species as long as the sample cocn is within the range of this kit.

MSDS

TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates