Human PLAU / uPA Protein, His Tag (activated by trypsin)

Source : 

Human PLAU, His Tag (activated by trypsin) (PLU-H5228) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Leu 431  (Accession # P00749-1).

 

Molecule : PLAU

 

Synonyms : Urokinase,PLAU,ATF,UPA,URK,u-PA,BDPLT5,QPD

 

Format : Powder

 

Category : Enzymes

 

Accession : NP_002649.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 15.3 kDa, 17.8 kDa and 29.2 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus.
The active form of Human PLAU is a disulfide-linked heterodimer composed of long chain A (Ser 21 - Phe 177) and chain B (Ile 179 - Leu 431) with calculated MW of 17.8 kDa and 29.2 kDa. The long

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 25 mM HEPES, 150 mM NaCl, pH7.5

 

Description :

Urokinase - type plasminogen activator is also known as PLAU and UPA, a serine protease with an extremely limited substrate specificity, cleaving the sequence Cys – Pro – Gly - Arg560 - Val561 – Val – Gly – Gly – Cys in plasminogen to form plasmin. uPA is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium.uPA and its receptor (uPAR) have been implicated in a broad spectrum of pathophysiological processes, including fibrinolysis, proteolysis, inflammation, atherogenesis and plaque destabilization, all of which are involved in the pathogenesis of MI (myocardial infarction).

 

References :

(1) Nagai M., et al., 1985, Gene 36:183.
(2) Jacobs P., et al., 1985, DNA 4:139.
(3) Harbeck, N. et al., 2002, Clin. Breast Cancer 3:196.