Human PH20 / SPAM1 Protein, His Tag

Source : 

Human PH20, His Tag (PH0-H5225) is expressed from human 293 cells (HEK293). It contains AA Leu 36 - Tyr 482 (Accession # P38567-1).

 

Molecule : PH20

 

Synonyms : SPAM1,PH-20,HYAL3,HYA1,HYAL1,HYAL5,SPAG15

 

Format : Powder

 

Category : Enzymes

 

Accession : NP_694859.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 53.0 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 53.0 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 50 mM Tris, 100 mM NaCl, pH7.5

 

Description :

Hyaluronidase PH-20 is also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N – acetyl – beta – D – glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

 

References :

(1) Lin Y., et al., 1993, Proc. Natl. Acad. Sci. U.S.A. 90:10071-10075.
(2) Gmachl M., et al., 1993, FEBS Lett. 336:545-548.
(3) Arming S., et al., 1997, Eur. J. Biochem. 247:810-814.