Human PBK / PDK / PDZ binding kinase / TOPK ELISA Pair Set

分子 : PBK

 

保存條件:

Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

 

 

線性範圍 : 62.5-4000

 

包被抗體 : 0.3 mg/mL of mouse anti-PBK monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 10729-MM10)

 

檢測抗體: 0.5 mg/mL mouse-anti-PBK monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10729-MM05)

 

標準品 : Each vial contains 40 ng of recombinant PBK. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 4 ng/mL is recommended.

 

生物素交聯HRP:

 

靈敏度 : The minimum detectable dose of Human PBK / TOPK was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

 

特異性 :

 

檢測原理 : The human PBK / TOPK ELISA Pair Set is for the quantitative determinationof human PBK / TOPK.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is asolid phase sandwich ELISA (Enzyme-Linked ImmunosorbentAssay). It utilizes a monoclonal antibody specific for PBK / TOPK coated on a 96-well plate. Standards and samples are added tothe wells, and any PBK / TOPK present binds to the immobilizedantibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-PBK / TOPKmonoclonal antibody is thenadded, producing an antibody-antigen-antibody "sandwich". The wells are againwashed and TMB substrate solution is loaded, which produces color in proportionto the amount of PBK / TOPK present in the sample. To end theenzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

 

檢測限: 62.5 pg/ml

 

血清樣本檢測範圍 :