Human P-Selectin / CD62P Protein, His Tag

Source : 

Human P-Selectin, His Tag (SEP-H52E4) is expressed from human 293 cells (HEK293). It contains AA Trp 42 - Ala 771 (Accession # AAN06828).

 

Molecule : P-Selectin

 

Synonyms : P-Selectin,CD62P,SELP,GMP-140

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : AAN06828

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 81.8 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 81.8 kDa. The protein migrates as 110-125 kD under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

P-selectin (SELP) is also known as CD62 antigen-like family member P, granule membrane protein 140 (GMP-140), leukocyte-endothelial cell adhesion molecule 3 (LECAM3) and platelet activation dependent granule-external membrane protein (PADGEM). SELP functions as a cell adhesion molecule (CAM) on the surfaces of activated endothelial cells, which line the inner surface of blood vessels, and activated platelets. In unactivated endothelial cells, it is stored in granules called Weibel-Palade bodies. In unactivated platelets SELP is stored in α-granules. The primary ligand for SELP is P-selectin glycoprotein ligand-1 (PSGL-1) which is expressed on almost all leukocytes, although P-selectin also binds to heparan sulfate and fucoidan. Furthermore, SELP has a functional role in metastasis of tumor similar to E-selectin, which can help cancer cells invade into bloodstream for metastasis and provided locally with multiple growth factors respectively.

 

References :

(1) Pouyani T., et al., 1995, Cell 83:333-343.
(2) Wilkins P.P., et al.,1995, J. Biol. Chem. 270:22677-22680.
(3) Kerr S.C., et al., 2008, J. Immunol. 181:1480-1490.