Human NT3 ELISA Kit (2 plates)

ZZZZ_Status (Active, Disc) : Discontinued

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

NT-3; NTF3; Neurotrophin-3; HDNF; Nerve growth factor 2; NGF-2;

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=826

 

Description (Long Description) : 

The human NT3 Kit is a sandwich ELISA. The capture antibody is a monoclonal anti-NT3 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known NT3 concentration are added to these wells and allowed to complex with the bound NT3 antibody. A biotinylated NT3 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of NT3 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human NT3 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

 

Expiry_Date : 

This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :The standard and immunogen in this assays is produced in and purified from an insect expression system consisting of amino acids Y139-T257

 

Kit_Components : The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, detection reagents, substrate buffer and detailed protocols.

 

MSDS (File name) :