Human NME1 / NDKA / NM23-H1 Protein, His Tag

Source : 

Human NME1, His Tag (NM1-H5147) is expressed from E.coli cells. It contains AA Ala 2 - Glu 152 (Accession # AAH00293).

 

Molecule : NME1

 

Synonyms : NME1,NDP kinase A,NDPA,GAAD,NM23-H1

 

Format : Powder

 

Category : Other Recombinant Proteins

 

Accession : AAH00293

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 18.0 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 18.0 kDa. The protein migrates as 21 kDa under reducing (R) condition (SDS-PAGE).

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Nucleoside diphosphate kinase A, a member of the NDK family, is also known as NME1, NDP kinase A (NDPA), granzyme A-activated DNase (GAAD), metastasis inhibition factor nm23 (NM23-H1) and tumor metastatic process-associated protein. NME1 plays a major role in the synthesis of nucleoside triphosphates other than ATP. NME1 is also involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Furthermore, NME1 is required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, NME1 works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.

 

References :

(1) Rosengard A.M., et al., 1989, Nature 342:177-180.
(2) Fan Z., et al., 2003, Cell 112:659-672.
(3) Min K., et al., 2002, Proteins 46:340-342.