Human Neuron Specific Enolase (NSE) ELISA Kit

SpeciesReactivity

Human

Samples

Serum. Other fluids not optimized.

KitContents

1. Streptavidin coated microwell strip plate (96 wells), Cat. # 0051 1 plate 2. Human NSE Std. A or sample diluent, 0 ug/L, 11 ml, Cat. # 052 1 vial 3. Human NSE Std. B, 0.75 ml, 5 ug/L, Cat. # 053 1 Vial 4. Human NSE Std. C, 0.75 ml, 25 ug/L, Cat. # 054 1 Vial 5. Human NSE Std. D, 50 ug/L, 0.75 ml, Cat. # 055 1 Vial 6. Human NSE Std. E, 100 ug/L, 0.75 ml, Cat. # 056 1 Vial 7. Human NSE Std. F, 200 ug/L, 0.75 ml, Cat. # 057 1 Vial 8. Biotinylated anti-NSE capture antibody, 11 ml Cat. # 058 1 bottle 9. Anti-NSE-HRP Conjugate, 11 ml, Cat. # 059 1 bottle 10. Wash Buffer (20X); 50 ml, Cat# WB-050 1 bottle 11. HRP substrate Soln A, Cat. # 050SA 1 bottle 12. HRP substrate Soln B, Cat. # 050SB 1 bottle 13. Stop solution, 10 ml, Cat. # T-10 1 bottle 14. Instruction Manual, M-050 1

Form

Stds=0-200 ug/L ; Sample=25 ul; Sensitivity= 1 ug/L; 150 min assay; Quantitative, Sandwich, 96 tests

SpCrossreactivity

The antibodies used in this kit are specific for gamma-subunit of NSE (¿-NSE). The antibodies do not detect NSE with other subunits. The kit is designed for human NSE. Antibody crossreactivity or the use of this kit in other species (mouse, rat, etc) has not been studied.

MSDS

TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates