Human Nectin-3 / CD113 Protein, His Tag

Source : 

Human Nectin-3, His Tag (PV3-H52E4) is expressed from human 293 cells (HEK293). It contains AA Gly 58 - Asp 400 (Accession # Q9NQS3).

 

Molecule : Nectin-3

 

Synonyms : CD113,Nectin-3,PVRL3

 

Format : Powder

 

Category : Immune Checkpoint Proteins

 

Accession : Q9NQS3

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 39.6 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 39.6 kDa. The protein migrates as 60-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Poliovirus receptor-related 3 (PVRL3), also known as nectin-3 and CD113, is a human protein of the immunoglobulin superfamily which forms part of adherens junctions. Nectins are immunoglobulin-like adhesion molecules that interact with afadin (AF6; MIM 159559). Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin system in epithelial cells. PVRL3 plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Furthermore, PVRL3 induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation.

 

References :

(1) Mueller S., et al., 2003, J. Biol. Chem. 278:31251-31260.
(2) Yu X., et al., 2009, Nat. Immunol. 10:48-57.