Human Mesothelin / MSLN (296-580) Protein, His Tag

Source : 

Human Mesothelin (296-580), His Tag (MSN-H5223) is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272).

 

Molecule : Mesothelin

 

Synonyms : MSLN,Mesothelin,MPF

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : AAH09272

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 33.0 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33.0 kDa. The protein migrates as 34-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

 

References :

(1) Chang K., et al., 1996, Proc. Natl. Acad. Sci. U.S.A. 93:136-140.
(2) Yamaguchi N., et al., 1994, J. Biol. Chem. 269:805-808.
(3) Rump A., et al., 2004, J. Biol. Chem. 279:9190-9198.
(4) Ma J., et al., 2012, J. Biol. Chem. 287:33123-33131.