Human MCAM / CD146 Protein, His Tag

Source : 

Human MCAM, His Tag (CD6-H5229) is expressed from human 293 cells (HEK293). It contains AA Val 24 - Gly 559 (Accession # NP_006491.2).

 

Molecule : MCAM

 

Synonyms : MCAM,CD146,MUC18

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : NP_006491.2

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 60.5 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 60.5 kDa. The protein migrates as 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

CD146, also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. As a member of the Immunoglobulin superfamily, It is expressed on chicken embryonic spleen and thymus, activated human T cells, endothelial progenitors such as angioblasts and mesenchymal stem cells, and strongly expressed on blood vessel endothelium and smooth muscle.
CD146 has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. The CD146+ T cells display an immunophenotype consistent with effector memory cells and have a distinct gene profile from the CD146- T cells.
As a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor,CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis or immune recognition.

 

References :

(1) Kuske MD, et al.,1999, Cytogenet. Cell Genet. 87 (3-4): 258.
(2) Elshal MF, et al., 2005, Blood. 106 (8): 2923–4.
(3) Elshal MF, et al., 2007, BMC Immunol. 8: 29.
(4) Covas DT, et al., 2008, Exp. Hematol. 36 (5): 642–54.
(5) Russell KC, et al., 2010, Stem Cells 28 (4): 788–98.