Human M-CSF/CSF1 ELISA Kit

分子 : CSF1

 

抗原貨號 : 11792-HNAH

 

產品介绍 : For the quantitative determination of Human M-CSF/CSF1 concentration in serum, cell culture supernates and urine.

 

線性範圍: 11.72-750

 

包被抗體類型 : rabbit mAb

 

檢測抗體類型 : rabbit mAb

 

靈敏度: 11.72

 

天然樣本測定含量: The average concentration of Human MCSF in 10 normal human serum is 1.47 +/- 0.50 ng/mL ranging from 0.94 to 2.54 ng/mL.The average concentration of MCSF in 4 normal human urine is 0.30 +/- 0.48 ng/mL ranging from 0.016 to 1.01 ng/mL. Human peripheral blood mononuclear cells （1 x 10E6 cells/mL） were cultured in RPMI supplemented with 10% fetal calf serum, 50 μM β-mercaptoethanol,2 mM L-glutamine, 100 units/mL penicillin, 100 μg/mL streptomycin sulfate, stimulated with 10 μg/mL PHA for 5 days. A

 

特異性 : This assay recognizes both recombinant and natural Human MCSF. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

 

干擾 : Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human MCSF control were assayed for interference. No significant interference was observed.

 

檢測原理: Solid Phase Sandwich ELISA

 

試劑盒组分 :

1. 96 well microplate coated with Capture Antibody

2. Detection Antibody conjugated to HRP

3. Standards

4. Wash Buffer Concentrate

5. Dilution Buffer Concentrate

6. Color Reagent A

7. Color Reagent B

8. Stop Solution