Human Luteinizing Hormone (LH) ELISA Kit

SpeciesReactivity

Human

Samples

Serum. Also used in human saliva (see published refs). Other fluids not optimized.

KitContents

1. Anti-human LH coated microwell strip plate (96 wells), 1 Plate 2. LH Sample Diluent , 0 mIU/ml 11 ml, 3. LH Calibrator, 10 mIU/ml, 0.75 ml 4. LH Calibrator, 20 mIU/ml, 0.75 ml 5. LH Calibrator, 40 mIU/ml 0.75 ml 6. LH Calibrator, 100 mIU/ml 0.75 ml 7. LH Calibrator, 200 mIU/ml, 0.75 ml Note: Standards are calibrated to WHO 2nd IS 80/552. 8. Anti-hLH-Enzyme Conjugate, 11 ml, 1 bottle 9. Solution A, 11 ml, Cat# 100SA 1 bottle 10. Solution B, 11 ml, Cat# 100SB 1 bottle 11. Stop solution, 10 ml, Cat. # T-10 1 bottle 12. Instruction Manual, M-100 1

Form

Stds=0-200 mIU/ml ; Sample=25 ul; Sensitivity= 2 mIU/ml; 40 min assay; Quantitative, Sandwich, 96 tests

SpCrossreactivity

The specificity of LH ELISA kit was determined by measuring interference from high concentrations of hFSH (up to 200 mIU/ml), hTSH (up to 50 uIU/ml), and hCG (25 mIU/ml). These hormones produced color intensity equal to 5, 2.5, and 16 mIU/ml, respectively. Human LH ELISA kit species crossreactivity with LH from other species (mouse, rat, and monkey) has not been established.

MSDS

TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates