Human Lox-1/OLR1 ELISA Kit (1 Plate)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Oxidized low-density lipoprotein receptor 1 (Lox-1/OL1)

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

ELISA. The purpose of this kit is the in-vitro quantitative determination of human OLR1 in samples such as cell culture supernatants, cell lysates, serum and plasma (heparin and EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info : LOX-1/OLR1, is a receptor protein that in humans is encoded by the OLR1 gene. LOX-1 mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis (Uniprot).

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1690

 

Description (Long Description) : 

The Human OLR1 ELISA kit is a sandwich ELISA. The capture antibody is a mouse monoclonal OLR1 antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known OLR1 concentration are added to these wells and allowed to complex with the bound OLR1 antibody. A biotinylated OLR1 goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of OLR1F present in the samples. The purpose of this kit is the in-vitro quantitative determination of human OLR1 in samples such as cell culture supernatants, serum and plasma (heparin, EDTA and Citrate). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

 

Expiry_Date : 

6 months after purchase if unopened

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :The standard and immunogen for this kit is recombinant human OLR1. The expression system is NSO; Immunogen sequence: S61-Q273

 

Kit_Components : The ELISA kit box contains 1 x 96-well pre-coated strip plate, human OLR1 protein standards, human OLR1 biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample diluent, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.

 

MSDS (File name) :http://www.biosensis.com/pdf_MSDS.php?products_id=1690