Human Leptin

SOURCE: E. coli

PURITY: >98% by SDS-PAGE and HPLC analyses Endotoxin level is <0.1 ng per ug of Leptin.

MOL. WEIGHT: 16.0 kDa

FORM: Sterile filtered and lyophilized

RECONSTITUTION: Centrifuge the vial prior to opening. Add 15 mM sterile HCl (0.5 ml/1 mg vial or 5 ml/10 mg vial) to the vial. After the protein is completely dissolved, add 7.5 mM sterile NaOH (0.3 ml/1 mg vial or 3 ml/10 mg vial) to bring the pH to approximately 5.2.

STORAGE CONDITIONS: Stored at -20oC or lower. Reconstituted Leptin should be stored in working aliquots at -20oC. If precipitation occurs, centrifuge briefly to remove insolubles. The active form of leptin should be in solution.

Leptin (Anti-Obesity Protein) is the protein product of the ob (obese) gene in mice and seems to be involved in appetite control. The ob gene is expressed in adipose tissue and is thought to regulate the body's fat stores. Mice with the ob/ob genotype also develop a form of diabetes similar to type II (non-insulin dependent) diabetes. Human Leptin is a 16 kDa protein containing 146 amino acid residues.

DESCRIPTION:

BIOLOGICAL ACTIVITY: BioVision's human Leptin has been shown to be biologically active in two different mouse obesity models, ob/ob and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5 ug Leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.

USAGE:

FOR RESEARCH USE ONLY! NOT TO BE USED IN HUMANS.