Human Leptin /Obese protein / Obesity factor ELISA Kit (1 plate)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Leptin; Obesity factor; Lep; Ob; Obese protein;

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1368

 

Description (Long Description) : 

The human Leptin Kit is a sandwich ELISA. The capture antibody is a monoclonal human Leptin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Leptin concentration are added to these wells and allowed to complex with the bound Leptin antibody. A biotinylated human Leptin monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Leptin present in the samples. The purpose of this kit is the in-vitro quantitative detection of human leptin in cell culture supernatants, serum, and heparin or EDTA treated plasma samples only when used as directed. Not recommended for tissue lysate detection of leptin. .

 

Expiry_Date : 

This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :The standard used in this kit is recombinant human leptin amino acids 22-167 produced in and purified from E. coli.

 

Kit_Components : The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.

 

MSDS (File name) :