Human Lactate Dehydrogenase A Inhibitor Screening Kit (Colorimetric)

Alternate Name : LDH Inhibitor Screening Kit

Tag Line :

Industry's first kit to screen/study/characterize potential inhibitors of LDH

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method - Absorbance (OD = 450 nm).
• Applications-Screening/characterizing/studying potential inhibitors of Lactate Dehydrogenase A

Detection Method: Absorbance (OD = 450 nm).

Features & Benefits:

• Simple and sensitive assay.
Fast protocol - can be performed in 30 min.
HTP adaptable.

Description:

The human oxidoreductase Lactate Dehydrogenase A (LDHA) (EC:1.1.1.27) is a key enzyme in lactic acid metabolism. It interconverts pyruvate and NADH to lactate and NAD+ respectively. In mammals, LDHA exists as a tetramer and is primarily found in skeletal muscle tissues. LDHA is a common marker to assay for tissue damage and is also known to promote tumor proliferation in breast, lung, and pancreatic cancer patients. In BioVision’s LDHA Inhibitor Screening Kit, LDHA catalyzes the conversion of lactate to an intermediate that interacts with a probe to develop a color that can be measured at OD 450 nm. In the presence of a LDHA specific inhibitor, the reaction is impeded. A LDHA Inhibitor Control is included to compare the efficacy of the sample inhibitors. The assay is high-throughput adaptable and can be completed in less than 1 hr