Human KIT / c-KIT / CD117 ELISA Kit

分子 : KIT

 

抗原貨號 : 11996-H08H

 

產品介绍 : For the quantitative determination of Human KIT / c-KIT / CD117 concentration in serum.

 

線性範圍: 39-2500

 

包被抗體類型 : rabbit mAb

 

檢測抗體類型 : mouse mAb

 

靈敏度: 9.4

 

天然樣本測定含量: The average concentration of human KIT detected in 10 human serum from apparently healthy people is 43.6 +/-14.3ng/mL ranging from 28.8 to 66.7 ng/mL.

 

特異性 : This assay recognizes both recombinant and natural Human KIT. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

 

干擾 : Preparations of FLT3、MCSFR、PDGFRα、PDGFRβ、VEGFR1、VEGFR2 and VEGFR3 at 25 ng/mL in a mid-range recombinant human KIT control were assayed for interference. No significant interference was observed.Recombinant human SCF does not cross-react in this assay but does interfere at concentrations greater than 5 ng/mL.

 

檢測原理: Solid Phase Sandwich ELISA

 

試劑盒组分 :

1. 96 well microplate coated with Capture Antibody

2. Detection Antibody conjugated to HRP

3. Standards

4. Wash Buffer Concentrate

5. Dilution Buffer Concentrate

6. Color Reagent A

7. Color Reagent B

8. Stop Solution