Human Junctional Adhesion Molecule A ELISA Kit

分子 : F11R

 

抗原貨號 : 10198-H08H

 

產品介绍 : For the quantitative determination of Human Junctional Adhesion Molecule A / JAM-A concentration in serum, cell culture supernates, and cell lysate.

 

線性範圍: 7.8-500

 

包被抗體類型 : mouse mAb

 

檢測抗體類型 : mouse mAb

 

靈敏度: 4.1

 

天然樣本測定含量: The average concentration of JAM-A in 8 normal human serum is 2.85+/-0.33 ng/mL ranging from 2.38 to 3.29 ng/mL. The concentration of Human JAM-A detected in a Hela cell lysate was 918.01 pg/mL.The Hela cell lysate' total protein concentration was about 9.3 mg/mL . HepG2 cells (0.2 x 10E5 cells/mL) were cultured 3 days. An aliquot of the cell culture supernate was removed, assayed for levels of natural JAM-A, and measured 265.01 pg/mL.

 

特異性 : This assay recognizes both recombinant and natural Human JAM-A.

 

干擾 :

 

檢測原理: Solid Phase Sandwich ELISA

 

試劑盒组分 :

1. 96 well microplate coated with Capture Antibody

2. Detection Antibody conjugated to HRP

3. Standards

4. Wash Buffer Concentrate

5. Dilution Buffer Concentrate

6. Color Reagent A

7. Color Reagent B

8. Stop Solution