Human Interleukin-6 (IL-6)/Interferon beta-2 (IFN-beta-2/B-cell stimulatory factor 2 (BSF-2) ELISA Kit (2 plates)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

IL-6; Interleukin-6; B-cell stimulatory factor 2; BSF-2; Interferon beta-2; Hybridoma growth factor; CTL diffenrentiation factor; CDF; IL6; IFNB2;

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=790

 

Description (Long Description) : 

The human Interleukin 6 (IL-6) kit is a sandwich ELISA. The capture antibody is a monoclonal human IL-6 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known IL-6 concentration are added to these wells and allowed to complex with the bound IL-6 antibody. A biotinylated human IL-6 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-6 present in the samples. The purpose of this kit is For quantitative detection of human IL-6 in cell culture supernatants, serum, and heparin, EDTA or citrate treated plasma samples only when used as directed This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

 

Expiry_Date : 

This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :The standard product used in this kit is recombinant human IL-6, consisting amino acids 29-212 expressed in and purified from an E. coli expression system

 

Kit_Components : The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, detection reagents, substrate buffer and detailed protocols.

 

MSDS (File name) :