Human Interleukin 1 beta ELISA Kit (1 plate)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Interleukin-1 beta; IL-1 beta; Catabolin; IL1B; IL1F2; IL1-beta;

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1344

 

Description (Long Description) : 

The human Interleukin 1 beta (IL-1b) Kit is a sandwich ELISA. The capture antibody is a monoclonal human IL-1b antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known IL-1b concentration are added to these wells and allowed to complex with the bound IL-1b antibody. A biotinylated anti-human IL-1b antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-1b present in the samples. The purpose of this kit is the in-vitro quantitative determination of human IL-1b in samples such as sera, plasma, and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

 

Expiry_Date : 

This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, A117-S269

 

Kit_Components : The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.

 

MSDS (File name) :