Human Integrin alpha 2b beta 3 (ITGAIIb&ITGB3) Heterodimer Protein, His Tag&Tag Free

Source : 

Human ITGAIIb&ITGB3 Heterodimer Protein, His Tag&Tag Free (IT3-H52W8) is expressed from human 293 cells (HEK293). It contains AA Leu 32 - Arg 993 (ITGAIIb) & Gly 27 - Asp 718 (ITGB3) (Accession # P08514-1 (R887L) (ITGAIIb) & P05106-1 (ITGB3)).

 

Molecule : Integrin alpha 2b beta 3

 

Synonyms : Integrin alpha 2b beta 3,ITGAIIb&ITGB3

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : NP_000410.2 (R887L) (ITGAIIb) & AAI27667.1 (ITGB3)

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 111.4 kDa (ITGAIIb) & 81.8 kDa (ITGB3)

 

Characteristics :

Human ITGAIIb&ITGB3 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAIIb and ITGB3, has a calculated MW of 111.4 kDa (ITGAIIb) and 81.8 kDa (ITGB3). Subunit ITGAIIb is fused with an acidic tail at the C-terminus and followed by a po

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 50 mM Tris, 150 mM NaCl, pH7.5

 

Description :

Integrin alpha IIb beta 3 exist in a conformational equilibrium clustered around four main states. These conformations range from a compact bent nodule to two partially extended intermediate conformers and finally to a fully upright state. Activation of blood platelets by physiological stimuli at sites of vascular injury induces inside-out signaling, resulting in a conformational change of the prototype Integrin alpha IIb beta 3 from an inactive to an active state competent to bind soluble fibrinogen. Furthermore, ligand occupancy of Integrin alpha IIb beta 3 outside-in signaling and additional conformational changes of the receptor, leading to the exposure of extracellular neoepitopes termed ligand-induced binding sites (LIBS), which are recognized by anti-LIBS monoclonal antibodies.

 

References :

(1) Leisner TM., et al., 1999, J. Biol. Chem. 274:12945-12949.
(2) Basani RB., et al., 1996, Blood. 88:167-173.