Human Insulin R / CD220 (28-944) Protein, His Tag (SPR verified)

Source : 

Human Insulin R (28-944), His Tag (SPR verified) (INR-H52Ha) is expressed from human 293 cells (HEK293). It contains AA His 28 - Lys 944 (Accession # P06213-2).

 

Molecule : Insulin R

 

Synonyms : INSR,Insulin receptor,IR,CD220

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : NP_000199.2

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 106.5 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 106.5 kDa. The protein migrates as 115-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Insulin receptor (INSR) is also known as CD antigen CD220, which can be cleaved into the following 2 chains: Insulin receptor subunit alpha and Insulin receptor subunit beta. INSR is a tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains carry the insulin-binding regions, while the beta chains carry the kinase domain. Forms a hybrid receptor with IGF1R, the hybrid is a tetramer consisting of 1 alpha chain and 1 beta chain of INSR and 1 alpha chain and 1 beta chain of IGF1R. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII). Isoform Short of INSR has a higher affinity for IGFII binding. When present in a hybrid receptor with IGF1R, INSR binds IGF1.

 

References :

(1) Pandini G., et al., 2002, J. Biol. Chem. 277:39684-39695.
(2) Slaaby R., et al., 2006, J. Biol. Chem. 281:25869-25874.
(3) Menting J.G., et al., 2013, Nature 493:241-245.
(4) Kadowaki T., et al., 1988, Science 240:787-790.