Human IL4/IL-4/Interleukin-4 ELISA Kit

分子 : IL4

 

抗原貨號 : 11846-H08H

 

產品介绍 : For the quantitative determination of Human IL4/IL-4/Interleukin-4 concentration in cell culture supernates.

 

線性範圍: 10.94-700

 

包被抗體類型 : mouse mAb

 

檢測抗體類型 : mouse mAb

 

靈敏度: 2.54

 

天然樣本測定含量: Human peripheral blood lymphocytes (PBLs) were isolated fromwhole blood using ficol-hypaque and passed through an R&D Systems T-Cell enrichmentcolumn. Eluted T-Cells were cultured at 2 x 106/mL in DMEM with 10% fetal calf serum,5  g/mL PHA, and 10 ng/mL rhIL-2. After 6 days of culture, cells were changed into DMEM with 10% fetal calf serum, 10 ng/mL PMA, 1  g/mL Ca2+ ionophore, and 5  g/mL PHA.Supernates were collected after 20 hours of stimulation and measured 70 pg/mL

 

特異性 : This assay recognizes both recombinant and natural Human IL4. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

 

干擾 :

 

檢測原理: Solid Phase Sandwich ELISA

 

試劑盒组分 :

1. 96 well microplate coated with Capture Antibody

2. Detection Antibody conjugated to HRP

3. Standards

4. Wash Buffer Concentrate

5. Dilution Buffer Concentrate

6. Color Reagent A

7. Color Reagent B

8. Stop Solution