Human IL2 ELISA Kit

分子 : IL2

 

抗原貨號 : 11848-HNAE

 

產品介绍 : For the quantitative determination of Human IL2 concentration in serum, cell culture supernates and plasma.

 

線性範圍: 18.75-1200

 

包被抗體類型 : mouse mAb

 

檢測抗體類型 : mouse mAb

 

靈敏度: 8.61

 

天然樣本測定含量: The average concentration of IL2 in 10 normal human serum is 14.00+/-0.06 pg/mL ranging from ND to 181.69 pg/mL. The average concentration of Human IL2 in 10 normal human plasma is 2.00 +/- 40 pg/mL ranging from ND to 102.67 ng/mL.
PBMC (2 x 10E6 cells/mL) were cultured in 1640 supplemented with 10% bovine calf serum. The cells were cultured stimulated with 2 μg/ml CD3 and CD28 for 3days. Aliquots of the cell culture supernates were removed and assayed for levels of natural IL2 and measured 40 p

 

特異性 : This assay recognizes both recombinant and natural Human IL2. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

 

干擾 : Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human IL2 control were assayed for interference. No significant interference was observed.

 

檢測原理: Solid Phase Sandwich ELISA

 

試劑盒组分 :

1. 96 well microplate coated with Capture Antibody

2. Detection Antibody conjugated to HRP

3. Standards

4. Wash Buffer Concentrate

5. Dilution Buffer Concentrate

6. Color Reagent A

7. Color Reagent B

8. Stop Solution