Human IL10RB / CRFB4 ELISA Pair Set

分子 : IL10RB

 

保存條件:

Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

 

 

線性範圍 : 78.13-5000

 

包被抗體 : 0.5 mg/mL of mouse anti-IL10RB monoclonal antibody. Dilute to a working concentration of 2.0 μg/mL in CBS before coating. (Catalog: # 10945-MM01)

 

檢測抗體: 0.7 mg/mL rabbit anti-IL10RB polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to a working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.

 

標準品 : Each vial contains 110 ng of recombinant IL10RB. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 5000 pg/mL is recommended.

 

生物素交聯HRP:

 

靈敏度 : The minimum detectable dose of human IL10RB / CRFB4 was determined to be approximately 78.125 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

 

特異性 :

 

檢測原理 : The human IL10RB / CRFB4 ELISA Pair Set is for the quantitative determination of human IL10RB / CRFB4.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IL10RB /CRFB4 coated on a 96-well plate. Standards and samples are added to the wells,and any IL10RB / CRFB4 present binds to the immobilized antibody. The wells arewashed and a horseradish peroxidase conjugated rabbit anti-IL10RB polyclonalantibody is then added, producing an antibody-antigen-antibody "sandwich". Thewells are again washed and TMB substrate solution is loaded, which producescolor in proportion to the amount of IL10RB / CRFB4 present in the sample. Toend the enzyme reaction, the stop solution is added and absorbances of themicrowell are read at 450 nm.

 

檢測限: 78.125 pg/ml

 

血清樣本檢測範圍 :